Optimizing live-cell fluorescence imaging conditions to minimize phototoxicity
نویسندگان
چکیده
منابع مشابه
Reducing Incidental Fluorescence in Live Cell Imaging
Fluorescence staining is the prevailing image acquisition technique in cell biology research. A problem of this method is the incidental fluorescence, that is the total fluorescence collected by a camera pixel originating from all sources in the sample except for the targeted point. As a result, typical images exhibit blurry regions at the periphery of fluorescence stained cell structures. Gene...
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ژورنال
عنوان ژورنال: Journal of Cell Science
سال: 2020
ISSN: 1477-9137,0021-9533
DOI: 10.1242/jcs.242834